meaning. Most importantly, this six panel volcano plot can well

ate the information which is required for a better visualisation.

e 6.9 shows such an example of the six-panel volcano plot for the

cancer data, where 29 down-regulated DEGs and 72 up-regulated

re clearly displayed if the threshold of base two logarithm of fold

was one and the critical p value was 0.01. In other words, DEGs

ermined based on both statistical and biological significance as

ser-defined thresholds.

g. 6.9. A six-panel volcano plot generated for the prostate cancer data.

panel of a six-panel volcano plot can be well-interpreted. For this

cancer data set, the upper-left panel was composed of down-

DEGs, which were 29. The upper-right panel was composed of

gulated DEGs. The upper-middle panel was composed of genes

ere statistically significant but biologically insignificant. The

t panel was composed of genes which were down-regulated in

biological significance but statistically insignificant. The lower-

el was composed of genes which were up-regulated in terms of

l significance but statistically insignificant. The lower-middle

as composed of genes which were both biologically and

ly insignificant. The R code of drawing a six-panel volcano plot

quested from the author via an email.